This checklist has been compiled to provide individuals involved in HIV and AIDS prevention programmes and policies with a tool to assess whether their work is sensitive to the needs of women, men, girls, and boys. The checklist can be used in the development and/or implementation of an HIV and AIDS prevention programme or policy. In addition, the checklist can be used to assess the gender sensitivity of organisations that implement such programmes and policies. It would be useful to programme and policy developers and health trainers